Autofluorescence of Bronchial Tissue
نویسندگان
چکیده
منابع مشابه
Reducing Tissue Autofluorescence
Introduction Immunofluorescence is a common technique in modern biology. It is a highly sensitive method used for the analysis of tissue samples with a fluorescence microscope. In this technique, antibodies are labeled with a fluorescent molecule in order to localize the antigen-antibody complex. Immunofluorescence (IF) can provide clear subcellular visualization of proteins, glycans, and small...
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Background: Immunofluorescence (IF) is one of the most important techniques where fluorochromes conjugated to antibodies are used to detect specific proteins or antigens. In tissue sections, autofluorescence (AF) can lead to poor quality images that impair assessment. The placenta is a pivotal extra-embryonic organ in embryo development, where trophoblasts make up a large proportion of the cell...
متن کاملAutofluorescence Detection of Bronchial Tumors With the D-Light/AF
We present a newly developed diagnostic system combining a conventional light source (white light mode and two different fluorescence excitation modes), a bronchoscope and optionally a highly sensitive camera (Baumgartner et al., Photochem. Photobiol. 1987; 46(5): 759-763). Routine diagnostics can be performed with the autofluorescence bronchoscopy (AFB) and the white light bronchoscopy (WLB) i...
متن کاملCigarette smoking, preinvasive bronchial lesions, and autofluorescence bronchoscopy.
STUDY OBJECTIVES Autofluorescence bronchoscopy (AFB), when used as an adjunct to standard white light bronchoscopy (WLB), enhances the bronchoscopist's ability to localize small neoplastic lesions, especially intraepithelial lesions. The current study was undertaken in order to define the population in which the rate of detection is higher using AFB. DESIGN AND PATIENTS Two hundred forty-four...
متن کاملCombined depth- and time-resolved autofluorescence spectroscopy of epithelial tissue.
A time-resolved confocal fluorescence spectroscopy system is built to measure the fine structure and localized biochemistry of epithelial tissue. It is found that the autofluorescence excited at 405 nm is sensitive to the cellular metabolism and can be used to sense the metabolic status of epithelial tissue. The decay of autofluorescence excited at 405 nm can be accurately fitted with a dual-ex...
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ژورنال
عنوان ژورنال: THE JOURNAL OF JAPAN SOCIETY FOR LASER SURGERY AND MEDICINE
سال: 1996
ISSN: 0288-6200
DOI: 10.2530/jslsm1980.17.1_75